Microbiological Potential and Cellulolytic Activity of Grey Forest Soil under Systematic Green Manure Use of Oilseed Radish
Abstract
Purpose. The aim of the research was to determine the effect of a long-term technological rotation cycle with systematic application of oil radish green manure on the structure of the microbiological complex and the cellulolytic activity of grey forest soil. Methods. The research was conducted from 2014 to 2025 at the experimental field of Vinnytsia National Agrarian University on grey forest soils with medium fertility potential. The experiment was replicated four times. The arrangement of variants was systematic in two tiers. The study aimed to assess the effectiveness and feasibility of long-term periodic (once every two years on the same field) green manuring of intermediate (summer) crops in crop rotation without including other cruciferous species, over a 12-year study cycle, compared with a control variant without green manuring and additional fertilization. Results. The technological feasibility of regulation aimed at optimizing the structure of ecological and functional groups of the grey forest soil microbiota was established, with an increase in the proportion of beneficial species by up to 30 %. A significant rise in cellulolytic activity was observed in the 0–30 cm soil profile, with an average annual gradient of 2.13 %/year and 2.38 %/year according to the functional criterion of linen tissue decomposition degree after 30 and 60 days of assessment, respectively, under consistent periodic use of oilseed radish green manure in the summer (intermediate) cultivation variant. Significant differences in the changes of soil cellulolytic activity were also identified under the green manure treatment compared with the control, with maximum values observed at a soil depth of 14–23 cm. Conclusions. The long-term use of oil radish as green manure in crop rotation proved to be effective in optimizing the microbiotic complex of grey forest soils. An improvement in the soil microbiocenosis structure was recorded: the total microbial biomass increased by up to 50 % over the full study cycle, while the proportion of beneficial microbiota grew by up to 30 % compared to the absolute (initial) control.
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