Grain quality formation of DEKALB maize hybrids depending on plant density and foliar application of micronutrients
Abstract
Purpose. To investigate the effect of plant density and foliar application of micronutrients on the crude protein content in the grain of DEKALB maize hybrids of different FAO maturity groups under the soil and climatic conditions of the Northern Steppe of Ukraine. Methods. Field method – for the establishment and conduct of the experiment; laboratory method – for determining crude protein content in the grain (Kjeldahl method); mathematical and statistical methods – for processing and interpreting the results. Statistical analysis was performed using Microsoft Office Excel andthe Agrostat software package. The reliability of the results was assessed using analysis of variance (ANOVA) and the least significant difference (LSD05) test. Results. The crude protein content in maize grain significantly depended on a combination of agrobiological and weather factors. It was established that the weather conditions of the growing season had a decisive influence: the highest protein levels were recorded in 2023, when moderate water deficit enhanced the synthesis of protein compounds in the grain. In contrast, during the drought year of 2024, pronounced hydrothermal stress during the critical phases of organogenesis led to a decrease in crude protein content, particularly in hybrids with lower stress tolerance. Among the studied hybrids, DKC 4391 (FAO 350) exhibited the highest and most stable crude protein content, indicating its strong genetic potential for protein accumulation even under variable moisture conditions. The optimal plant density for achieving a high protein potential was found to be 75–80 thousand plants/ha. Excessively low or high plant populations reduced photosynthetic efficiency and nutrient uptake due to unbalanced competition or inefficient use of the available rooting space, thus negatively affecting grain quality. A positive effect of foliar application of micronutrients on the accumulation of crude protein in maize grain was established. The use of Amino Ultra Maize resulted in the greatest increase in protein content compared to the control, especially under moderate water deficit, highlighting the effectiveness of amino acid-based micronutrient fertilizers in maize production technologies. Conclusions. The results substantiate the close interaction between agro-cli- matic conditions, hybrid genetics, plant spatial distribution in the field, and foliar fertilization in determining the protein composition of maize grain. The hybrid DKC 4391 (FAO350) achieved the highest crude protein content under the optimal plant density of 75–80 thousand plants/ha. The use of micronutrient fertilizers, particularly Amino Ultra Maize, proved to be an effective measure to improve this indicator.The obtained findings may serve as a basis for developing adaptive agronomic technologies to ensure stable grain quality under conditions of climate change and fluctuating water availability.
References
2. Li Ya., Zhang Р., Sheng W., Zhang Z., Rose R. J., Song Yo. Securing maize reproductive success under drought stress by harnessing CO2 fertilization for greater productivity. Frontiers in Plant Science. 2023. Vol. 14. Р. 1221095. DOI: https://doi.org/10.3389/fpls.2023.1221095
3. Guo J., Qu L., Hu Yі., Lu W., Lu D. Proteomics reveals the efects of drought stress on the kernel development and starch formation of waxy maize. BMC Plant Biology. 2021. Vol. 21. Р. 434. DOI: https://doi.org/10.1186/s12870-021-03214-z
4. Correndo A. A., Fernandez J. A., Vara Prasad P. V., Ciampitti І. А. Do Water and Nitrogen Management Practices Impact Grain Quality in Maize? Agronomy. 2021. Vol. 11(9). Р. 1851. DOI: https://doi.org/10.3390/agronomy11091851
5. Safian N., Naderi M. R., Torabi M., Soleymani A., Salemi H. R. Corn (Zea mays L.) and sorghum (Sorghum bicolor (L.) Moench) yield and nutritional quality affected by drought stress. Biocatalysis and Agricultural Biotechnology. 2022. Vol. 45. Р. 102486. DOI: https://doi.org/10.1016/j.bcab.2022.102486
6. Wang F., Wang L., Yu X., Gao J., Ma D., Guo H., Zhao H. Effect of Planting Density on the Nutritional Quality of Grain in Representative High-Yielding Maize Varieties from Different Eras. Agriculture. 2023. Vol. 13(9). Р. 1835. DOI: https://doi.org/10.3390/agriculture13091835
7. Ygzaw W., Ifie В. Е., Ribeiro Р. F., Adu G. B., Danquah E. Yi., Offei S. К., Tongoona Р. В. Optimizing Maize Yield With Hybrids Tolerant of High Plant Density in West and Central Africa. Plant Environ Interact. 2025. Vol. 6(2). Р. 70046. DOI: https://doi.org/10.1002/pei3.70046
8. Yang X., Li Q. Influence of Agronomic Practices on Maize Protein and Starch Contents. Field Crop. 2024. Vol. 7(3). Р. 158–170. DOI: https://doi.org/10.5376/fc.2024.07.0016
9. Asibi А. Е., Chai Q., Coulter J. А. Mechanisms of Nitrogen Use in Maize. Agronomy. 2019. Vol. 9(12). Р. 775. DOI: https://doi.org/10.3390/agronomy9120775
10. Ivanyshyn О., Khomina V., Pantsyreva Н. Influence of fertilization on the formation of grain productivity in different-maturing maize hybrids. Ukrainian Journal of Ecology. 2021. Vol. 11(3). Р. 262–269. DOI: https://doi.org/10.15421/2021_170
11. Мєлєшко І. О., Сидякіна О. В. Особливості мінерального живлення кукурудзи на зерно. Сучасна наука: стан та перспективи розвитку : матеріали V Всеукр. наук.-практ. конф. молодих вчених з нагоди Дня науки в Україні, 19 травня 2022 р. Херсон, 2022. С. 32–34.
12. Сидякіна О. В., Гамула Є. А. Мікродобрива у технологіях вирощування кукурудзи як елемент екологоорієнтованого та ресурсозберігаючого землеробства. Продовольча безпека України в умовах післявоєнного відновлення: глобальні та національні виміри. Міжнародний форум : доповіді учасників міжнародної науково-практичної конференції, 28–30 травня 2025 р. Миколаїв : МНАУ, 2025. С. 166–169.




