Modification of nutrient medium for in vitro cultivation of isolated organs, tissues and cells of Linum usitatissimum L. convar. elongatum

Abstract

Purpose. To develop a new nutrient medium on the basis of the Murashige and Skoog nutrient medium, which would be more suitable for the cultivation of Linum usitatissimum L. convar. elongatum in vitro and improved the process and result of cultivation was the aim of the study. Methods. Biotechnological (cultivation of isolated organs, tissues and cells in vitro, modification of nutrient medium, morphometric calculations), statistical (establishment of the arithmetic mean, errors of the sample mean, degree of significance of the difference according to the Student’s t-criterion). Results. Modification of Murashige and Skoog nutrient medium, which includes 440 mg/L (3 mМ) CaCl2 · 2Н2О, 370 mg/L (1.5 mМ) MgSO4 · 7H2O, 22.3 mg/L (100 μМ) MnSO4 · 4H2O, 8.6 mg/L (30 μМ) ZnSO4 · 7H2O, 0.83 mg/L (5 μМ) КJ, 0.25 mg/L (1 μМ) Na2МоО4 · 2H2O, 100 mg/L meso-inositol, and 2000 mg/L (25 mМ) NH4NO3, 2022 mg/L (20 mМ) KNO3, 136 mg/L (1 mМ) KH2PO4, 156 mg/L (1 mМ) NaH2PO4 · 2Н2O, 33.36 mg/L (0.12 mМ) FeSO4 · 7Н2О, complexed (chelated) 44.76 mg/L (0.12 mМ) EDTA-Na2, 9.27 mg/L (150 μМ) Н3ВО3, 5.3 mg/L (25 μМ) Na2SiO3 · 5H2O, 0.05 mg/L (0.2 μМ) CuSO4 · 5Н2О, 0.05 mg/L (0.2 μМ) CoCl2 · 6Н2О, 2.5 mg/L glycine, 0.25 mg/L nicotinic acid, 1 mg/L pyridoxine, 0.2 mg/L thiamine, 5 mg/L ascorbic acid, 4 mg/L succinic acid, 10,000–30,000 mg/L glucose, 8,000 g/L agar, is effective for increasing cultivation efficiency Linum usitatissimum L. convar. elongatum (flax) in vitro. Conclusions. Shoots of Linum usitatissimum L. convar. elongatum grown on a modified medium were characterized by more intensive growth, higher indicators of height traits, the number of internodes, the mass of callus formed on hypocotyl segments, the ability to organogenesis, the number of somaclones formed, a long period of ontogenesis, etc.